PAX8 Antibody

• Catalog Number: orb606932
• Category: Antibodies
• Description: Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the recombinant PAX8 antibody.
• UniProt ID: Q06710
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: PAX8/2774R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant PAX8 antibody as confirmation of integrity and purity.

IHC testing of FFPE human thyroid with PAX8 antibody. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min and allow to cool before testing.

IHC testing of FFPE human kidney with PAX8 antibody. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min and allow to cool before testing.