Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

SKU: orb749935

Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include Keratins 1, 3, 4, 5, 6, 8, 10, 14, 15, 16, and 19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the GITR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KRTL/1077 + KRTH/1076
• Immunogen: Recombinant human KRT77 and KRT76 protein were used as the immunogen for the pan Cytokeratin antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin paraffin rat stomach stained with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).

IHC: Formalin-fixed, paraffin-embedded rat oviduct with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).

Quick Database Links

UniProt

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