Nurr1 Rabbit pAb, FITC conjugated

SKU: orb464231

Description

Nurr1 antibody (FITC)

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test,IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nurr1
• Target: Nurr1
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Nur-ralated factor 1; Nuclear receptor-related factor 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; Regenerating liver nuclear receptor 1; RNR-1; SL-322; Nuclear orphan receptor HZF-3; T-cell nuclear receptor NOT; HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; NR4A2; NR4A2_HUMAN; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; Nurr 1; RNR 1; RNR1; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U-87MG. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (orb464231), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

Nurr1

References

Giuliano Bonfá, Giovanna Martino, View ORCID ProfileAssunta Sellitto, Antonio Rinaldi, Fabiana Tedeschi, View ORCID ProfileFabio Caliendo, Loris Melchiorri, Daniela Perna, Freddie Starkey, View ORCID ProfileEvangelos Nikolados, View ORCID ProfileFilippo Menolascina, View ORCID ProfileVelia Siciliano Design of novel synthetic promoters to tune gene expression in T cells bioRxiv, (2025)

PubMed:

Applications

IF

Reactivity

Human

Zhang, Wencan et al. Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced Treg differentiation via up-regulating Nr4a2 Sci Adv, 8, (2022)

PubMed: 35704583

Applications

FC

Reactivity

Mouse