Nucleophosmin Rabbit Polyclonal Antibody

SKU: orb1730

Description

Nucleophosmin Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nucleophosmin (21-120/259aa)
• Target: NPM1
• Molecular Weight: 33 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

B23; B23; MGC104254; NMP1; NMP1; NO38; NPM 1; NPM; NPM_HUMAN; NPM1; Nucleolar phosphoprotein B23; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin(nucleolar phosphoprotein B23 numatrin); Nucleophosmin; Nucleophosmin/B23.2; Nucleophosmin/nucleoplasmin family member 1; Nucleoplasmin Family Member 1; Nucleoplasmin Family Member 1; Numatrin; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398; TRK fused gene.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human SH-SY5Y cell lysates, Primary: Anti-Nucleophosmin (orb1730) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kDa, Observed band size: 37 kDa.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NPM1 Polyclonal Antibody, Unconjugated (orb1730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NPM1

UniProt

P06748