NKCC1/SLC12A2 Rabbit Polyclonal Antibody

SKU: orb157992

Description

NKCC1/SLC12A2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=3ug/test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NKCC1 (701-800/1212aa)
• Target: SLC12A2
• Molecular Weight: 132 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; mBSC2; NKCC1; S12A2_HUMAN; SLC12A2; sodium-potassium-chloride cotransporter 1; solute carrier family 12(sodium/potassium/chloride transporters) member 2; Solute carrier family 12 member 2; sy-ns.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-SLC12A2 antibody (orb157992), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 3 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

SLC12A2

UniProt

P55011