NAK/TBK1 Antibody

• Catalog Number: orb1535309
• Category: Antibodies
• Description: NAK/TBK1 Antibody
• Target: NAK / TBK1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-TIETSLQDIDSRLS, from the internal region of the protein sequence according to NP_037386.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 85kD band observed in lysates of cell line HeLa (calculated MW of 83.6kD according to NP_037386.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 24kD. This band was successfully blocked by incubation with the immunizing peptide.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TBK1, T2K, NAK, NF-kappa-B-activating kinase, NF-k
• Note: For research use only

TBK1 antibody (1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.