MSH6 Antibody

SKU: orb1261737

Description

MSH6 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Monkey, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human MSH6 (NP_000170.1).
• Target: MSH6
• Molecular Weight: Observed: 160kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

GTBP, GTMBP, HNPCC5, HSAP, p16DNA mismatch repair protein Msh6, G/T mismatch-binding protein, mutS protein homolog 6, mutS-alpha 160 kDa subunit, sperm-associated protein

Western blot analysis of extracts of various cell lines, using MSH6 antibody at 1:500 dilution.

Immunohistochemistry of paraffin-embedded human colon using MSH6 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using MSH6 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using MSH6 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MSH6 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence analysis of A549 cells using MSH6 antibody.

Quick Database Links

Gene Symbol

MSH6

UniProt

P52701