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| Catalog Number | orb392078 |
|---|---|
| Category | Proteins |
| Description | Recombinant mouse LGMN protein is produced by our mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus. |
| Reactivity | Mouse |
| Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCI, 150mM NaCl, 20% Glycerol, pH 8.0. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYVD HHHHHH |
| UniProt ID | O89017 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Alternative names | PRSC1; Legumain; Asparaginyl endopeptidase; Protea Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 19141 |
| Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
36.8 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
38.8 kDa | |
E.coli |
>90% as determined by SDS-PAGE. | |
51.42 kDa |
>75%, determined by SDS-PAGE | |
This protein contains (Val 18-Tyr 435) of mouse LGMN (NP_0353051) precursor was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |
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