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Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

SKU: orb347405

Description

Mouse IgG IgA IgM (H&L) antibody (Biotin)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:300,000, IHC: 1:1,500 - 1:15,000, WB: 1:3,000 - 1:30,000
ReactivityMouse
Application Notes
Anti-Mouse IgG IgA IgM Biotin Antibody has been tested by ELISA and is useful for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenMouse IgG IgA and IgM whole molecule
PurityThis product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal.

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
DisclaimerFor research use only

Alternative Names

Goat Anti Mouse IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Mouse IgG IgA IgM Biotin conjugated Antibody, Goat Anti-Mouse IgGAM Biotin conjugate

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed (orb347405)

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1 mg
$ 490.00
DispatchUsually dispatched within 5-10 working days
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