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Mouse CD274 protein
Catalog Number: orb391395
| Catalog Number | orb391395 |
|---|---|
| Category | Proteins |
| Description | Recombinant mouse CD274 protein is produced by our mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a Fc tag at the C-terminus. |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Reactivity | Mouse |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Target | CD274 |
| Entrez | 60533 |
| UniProt ID | Q9EP73 |
| Protein Sequence | FTITAPKDLY VVEYGSNVTM ECRFPVEREL DLLALVVYWE KEDEQVIQFV AGEEDLKPQH SNFRGRASLP KDQLLKGNAA LQITDVKLQD AGVYCCIISY GGADYKRITL KVNAPYRKIN QRISVDPATS EHELICQAEG YPEAEVIWTN SDHQPVSGKR SVTTSRTEGM LLNVTSSLRV NATANDVFYC TFWRSQPGQN HTAELIIPEL PATHPPQNRT VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K |
| Source | Human cells |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Alternative names | B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell dea Read more... |
| Note | For research use only |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
Immobilized Recombinant Mouse CD274 Protein at 2 μg/ml (100 μl/well) can bind Anti-CD274 antibody. The ED50 of Anti-CD274 antibody is 0.008 μg/ml.
- Item 1 of 4
Human PD-L1 Protein, mFc-His tag [orb689384]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 2
Mouse PD-L1 Protein, Fc Tag [orb257764]
Unconjugated
95%
51.4 kDa
Mouse PD-L1, Fc Tag (orb257764) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).
200 μg, 1 mg - Item 1 of 1
Mouse CD274 protein [orb391394]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μg - Item 1 of 2
Recombinant mouse PD-L1 protein, C-hFc (HEK293) [orb1516347]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
50 μg - Item 1 of 2
Recombinant mouse PD-L1 protein, C-His (HEK293) [orb1516348]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
50 μg
