Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody

SKU: orb108818

Description

Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody

Images & Validation

• Tested Applications: IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Rat
• Application Notes:
  This antibody can be used for gel supershift assays, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, this antibody recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: C5
• Immunogen: N-terminal fragment of human microphthalmia protein.
• Purification: Protein A/G Chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Concentration: See vial for concentration
• Disclaimer: For research use only

Alternative Names

MiTF

Immunohistochemical staining of tumor cells using Microphthalmia Transcription Factor antibody

Quick Database Links

UniProt

O75030