MART-1 / Melan-A Antibody

• Catalog Number: orb248353
• Category: Antibodies
• Description: Antibody A103 is specific for Melan-A (likely an abbreviation for 'melanocyte antigen'), also called MART-1, a unique antigen on melanoma cells recognized by CTLs (cytotoxic T lymphocytes), making it useful as a melanocyte differentiation marker. It is commonly used to identify metastatic tumors of melanocytic origin, as well as perivascular epithelioid cell tumors. Unlike another commonly used Melan-A / MART-1 antibody, M2-7C10, A103 also stains steroid hormone producing cells, making it valuable in identifying adrenocortical carcinomas. Note: this protein was identified in 1994 by two different labs, one naming it MART-1 (melanoma antigen recognized by T-cells) and the other Melan-A. Both names are commonly used in literature, but clone A103 antibody is generally referred to as being anti-Melan-A.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human protein was used as the immunogen for the Melan-A antibody.
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Due to differences in protocols and secondary antibody used, the Melan-A antibody may require titration for optimal performance.1. FFPE staining is enhanced by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: A103
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone A103). Expected molecular weight ~20 kDa with possible doublet.

SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone A103). Confirmation of Integrity and Purity of the Antibody.

IHC testing of FFPE human melanoma stained with Melan-A antibody (clone A103).