MART-1/Melan-A Antibody (Center)

• Catalog Number: orb1938018
• Category: Antibodies
• Description: MART-1/Melan-A Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q16655
• MW: 13157 Da
• Tested applications: FC, WB
• Dilution range: FC - 1:25, WB - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: MART1
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-MLANA Antibody (Center) at 1:2000 dilution + SK-MEL-3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

MART-1/Melan-A Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).

Overlay histogram showing A2058 cells (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.