LIG1 Antibody

• Catalog Number: orb626428
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DNA Ligase I
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ligase I, DNA, ATP-dependent
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: LIG1
• UniProt ID: P18858
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IP Result of anti-DNA Ligase I (IP: orb626428, 4 ug; Detection: orb626428 1:500) with HepG2 cells lysate 3200 ug.

HepG2 cells were subjected to SDS PAGE followed by western blot with orb626428 (LIG1 antibody) at dilution of 1:500.

Immunohistochemistry of paraffin-embedded human spleen using orb626428 (LIG1 antibody) at dilution of 1:100.