Goat anti-LIG1 / DNA ligase I Antibody

• Catalog Number: orb20267
• Category: Antibodies
• Description: Goat polyclonal antibody to LIG1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Rat
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 102
• Target: LIG1 / DNA ligase I
• Entrez: 3978
• Protein Sequence: RVREDKQPEQATTS
• RRID: AB_10752981
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti LIG1 antibody, anti ligase I, DNA, ATP-depend
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20267 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.