Keratin 6 Antibody / Cytokeratin 6

• Catalog Number: orb750218
• Category: Antibodies
• Description: In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM269
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: Amino acids GSSTIKYTTTS from the C-terminus of human keratin 6 was used as the immunogen for this Cytokeratin 6 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 6 antibody is available for research use only.
• UniProt ID: P02538, P04259, P48668
• Storage: Store the Cytokeratin 6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of fixed mouse RAW cells with Cytokeratin 6 antibody (clone SPM269, cyan) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (clone SPM269).

Flow cytometry testing of fixed mouse RAW cells with Cytokeratin 6 antibody (clone SPM269); Red=isotype control, Blue= Cytokeratin 6 antibody.