Keratin 6 Antibody / Cytokeratin 6

• Catalog Number: orb1825709
• Category: Antibodies
• Description: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16. Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests. Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the KRT6 antibody.
• UniProt ID: P02538
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Antibody Type: Primary Antibody
• Clone Number: KRT6/8267R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This KRT6 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human skin tissue with KRT6 antibody (clone KRT6/8267R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free KRT6 antibody (clone KRT6/8267R) as confirmation of integrity and purity.