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JAG1/Jagged 1 Antibody

SKU: orb1537801

Description

JAG1/Jagged 1 Antibody

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC, IHC-P, WB
Dilution rangeELISA (1:2500 - 1:10000), ICC, IF (1:100 - 1:500), IHC, IHC-P (5 µg/ml), WB (1:250 - 1:1000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: LS-B2442 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2442 was determined to be 5 ug/ml.

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenSynthetic peptide corresponding to aa 110-125 of human Jagged-1protein.
TargetJAG1 / Jagged 1
PurificationProtein A purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, pH 7.2, 0.01% Sodium Azide. PBS, pH 7.2, 0.01% Sodium Azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

JAG1, AGS, AHD, Alagille syndrome, CD339 antigen, HJ1, Jagged1, JAGL1, Jagged, Jagged 1, Protein jagged-1, AWS, CD339, Soluble protein jagged

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

JAG1/Jagged 1 Antibody (orb1537801)

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Available Sizes

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50 μg
$ 780.00
DispatchUsually dispatched within 2-3 weeks
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