ITGAX Antibody

SKU: orb1263555

Description

ITGAX Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This ITGAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1119-1145 amino acids from the C-terminal region of human ITGAX.
• Target: ITGAX
• Molecular Weight: 128 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150, CD11c, ITGAX, CD11C

Western blot analysis of ITGAX Antibody in MDA-MB231 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of ITGAX Antibody with paraffin-embedded human hepatocarcinoma. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Quick Database Links

Gene Symbol

ITGAX

UniProt

P20702

RefSeq:P20702