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Catalog Number | orb1230439 |
---|---|
Category | Proteins |
Description | IL-35 Recombinant Protein |
Target | IL12A |
Form/Appearance | Lyophilized |
Purity | ≥ 90% by SDS-PAGE gel and HPLC analyses. |
Protein Sequence | α Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS β Subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK |
UniProt ID | P29459 |
Source | HEK293 cells |
Biological Activity | Measured by its ability to inhibit proliferative activity of PBMCs. |
Storage | The recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | Interleukin-35 |
Note | For research use only |
Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. | |
73.9 kD | |
Human Cells |
> 98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). | |
CHO cells |
98.00% | |
102 kDa (predicted); 60 & 63 kDa (reducing conditions) |
98.00% | |
102.1 kDa (predicted); 66 kDa (reducing conditions) |
96.70% | |
48.3 kDa (predicted) |