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Catalog Number | orb1229883 |
---|---|
Category | Proteins |
Description | IL-35 Recombinant Protein |
Target | EBI3 |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS. |
Purity | > 98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
Protein Sequence | The human IL-35 complex composed of the Ebi3 subunit (aa 21-229) and the IL-12a (p35) subunit (aa 57-253) is fused through a polypeptide linker to the Fc portion of human IgG1. |
UniProt ID | Q4QNY4 |
Application notes | This recombinant proteins is for research use only. |
Source | CHO cells |
Biological Activity | Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells. |
Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
Alternative names | Interleukin-35 |
Note | For research use only |
NCBI | NP_000873, NP_005746 |
Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. | |
73.9 kD | |
Human Cells |
98.00% | |
102 kDa (predicted); 60 & 63 kDa (reducing conditions) |
98.00% | |
102.1 kDa (predicted); 66 kDa (reducing conditions) |
96.70% | |
48.3 kDa (predicted) |