Human Kallistatin (KAL) ELISA Kit

SKU: orb777949

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallistatin(KAL).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KAL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.43 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SERPINA4 ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI4 ELISA Kit, Serpin Peptidase Inhibitor Clade A Member 4 ELISA Kit, Tissue Kallikrein Inhibitor ELISA Kit, Kallikrein Binding Protein ELISA Kit, Peptidase inhibitor 4 ELISA Kit

Quick Database Links

UniProt

P29622