Human IL-4 Protein, His Tag

• Catalog Number: orb1149283
• Category: Proteins
• Description: Human IL-4, His Tag is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).
• Target: IL-4
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• Protein Sequence: NP_000580.1
• UniProt ID: P05112
• MW: 16.9 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. The protein migrates as 22-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-4, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) with a linear range of 0.5-16 ng/mL (QC tested).
• Expression Region: His 25 - Ser 153
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000580.1

Immobilized Human IL-4, His Tag (Cat. No. orb1149283) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag (Cat. No. orb545738) with a linear range of 0.5-16 ng/mL (QC tested).