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Catalog Number | orb1149287 |
---|---|
Category | Proteins |
Description | Mouse IL-4, His Tag is expressed from human 293 cells (HEK293). It contains AA His 21 - Ser 140 (Accession # P07750-1). |
Target | IL-4 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_067258.1 |
UniProt ID | P07750 |
MW | 15.4 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-4, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Mouse IL-4 R alpha, Fc Tag (Cat. No. ILR-M5252) with a linear range of 0.2-16 ng/mL (QC tested). |
Expression Region | His 21 - Ser 140 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_067258.1 |
Immobilized Mouse IL-4, His Tag (Cat. No. orb1149287) at 5 μg/mL (100 μL/well) can bind Mouse IL-4 R alpha, Fc Tag (Cat. No. orb334907) with a linear range of 0.2-16 ng/mL (QC tested).
> 90%, determined by SDS-PAGE | |
This protein contains the mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met. |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus and expressed from Baculovirus-Insect Cells. |
> 90% by SDS-PAGE. | |
Recombinant Mouse IL-4RA/IL-4 R alpha Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ile26-Arg233) of mouse IL-4RA/IL-4 R alpha (Accession #P16382) fused with an Fc tag at the C-terminus. |
> 95% by SDS-PAGE. | |
Recombinant Mouse IL-4 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (His Tag23-Ser140) of mouse IL-4 (Accession #NP_067258.1) fused with a 6��His Tag at the C-terminus. |