Human CLDN18.2 full length protein-VLP

SKU: orb1291767

Description

Human Full length Claudin 18.2 Protein-VLP

Key Properties

• Source: Mammalian
• Expression System: HEK293
• Target: CLDN18.2
• Reactivity: Human
• Tag: C-Flag Tag
• Molecular Weight: The Human Claudin 18.2 Protein has a predicted MW of 29 kDa.Due to PTM, the actual MW on SDS-PAGE gel is around 50kDa.

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Disclaimer: For research use only

Alternative Names

Claudin 18.2

ELISA plates were pre-coated with 0.5ug/per well purified human CLDN18.2 VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (Zolbetuximab biosililar; IMAB362) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for IMAB362 binding with CLDN18.2 VLP is 15.37 ng/ml.

FACS analysis of CLDN18.2 VLP.A. Negative Control 1: CLDN18.2 VLP were stained only with Goat anti-human IgG Fc-PE secondary antibody.B. Negative Control 2: Control VLP were stained with anti-CLDN18.2 antibody (Zolbetuximab biosimilar; IMAB362) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.C. Negative Control 3: CLDN18.2 VLP were stained with anti-BCMA antibody (an irrelevant antibody) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.D. CLDN18.2 VLP were stained with anti-CLDN18.2 antibody (Zolbetuximab biosimilar; IMAB362) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.

Quick Database Links

Gene Symbol

CLDN18.2

UniProt

P56856