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Human CD47 Protein, Fc Tag
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | CD47 |
| Reactivity | Human |
| Tag | C-Fc |
| Molecular Weight | 40.4 kDa |
| Expression Region | Gln 19 - Pro 139 |
| Purity | 95% |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH 7.5 |
| Disclaimer | For research use only |
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−Human CD47 Protein, mFc-His Tag [orb689369]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μgMouse CD47 [orb2994347]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.8 KDa. Observed: 60-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD47 [orb2994047]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 40.3 KDa. Observed: 48-65 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human CD47 [orb2994144]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.7 KDa. Observed: 60-70 KDa, reducing conditions
20 μg, 100 μgHuman CD47 [orb2994617]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 40.9 KDa. Observed: 60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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SDS-PAGE analysis of Human CD47 protein

Line graph illustrates about the sensitivity range of Human CD47 protein using different concentrations
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Protocol Information
Human CD47 Protein, Fc Tag (orb257295)
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