ACE2/ACEH protein-Fc (ACE2-FC-)

SKU: orb1945965

Description

ACE2/ACEH protein-Fc (ACE2-FC-)

Images & Validation

• Tested Applications: FC

Key Properties

• Species/Host: Rabbit
• Isotype: Other
• Reactivity: Human
• Tag: Fc tag
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

ACE2-2, ACEH, ACE2

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.