HSP70/HSC70 Antibody

SKU: orb1822511

Description

Mouse Anti-Human HSP70/HSC70 Monoclonal IgG1

Images & Validation

• Tested Applications: FC, ICC, IEM, IF, IHC, IP, WB
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  1 µg/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N27F3-4
• Immunogen: Human Recombinant HSP70/HSC70
• Target: HSP70/HSC70
• Molecular Weight: 73kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSPA1A, HSPA1B, HSPA1, HSPA8, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSC70, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP70/HSC70

UniProt

P0DMV9, P0DMV8

NCBI Gene ID

3303

RefSeq (Protein):NP_005336.3