HOMEZ Antibody

• Catalog Number: orb2636909
• Category: Antibodies
• Description: Homeodomain-containing proteins function as transcription factors that typically switch on cascades of other genes. Usually homeodomain proteins act in the promoter region of their target genes as complexes with other transcription factors, leading to much higher target specificity than a single homeodomain protein. HOMEZ (Homeobox and leucine zipper protein) is a 525 amino acid nuclear protein that contains 3 atypical homeodomains, 2 leucine zipper-like motifs, proline and serine-rich motifs and an acidic domain. Within homeodomain 2, it contains a putative nuclear localization signal. HOMEZ shares significant sequence similarity with mouse ZHX1 and sequences that are homologous to HOMEZ are restricted to vertebrates. Likely functioning as a transcription regulator, HOMEZ is ubiquitously expressed with highest levels found in in ovary, testis, kidney, fetal lung and kidney.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-HOMEZ-1A5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human HOMEZ protein was used as the immunogen for the HOMEZ antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HOMEZ antibody is available for research use only.
• UniProt ID: Q8IX15
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HOMEZ antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of PFA-fixed human HeLa cells with HOMEZ antibody (blue, clone PCRP-HOMEZ-1A5), and isotype control (red).

IHC staining of FFPE human lymph node tissue with HOMEZ antibody (clone PCRP-HOMEZ-1A5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using HOMEZ antibody (clone PCRP-HOMEZ-1A5). These results demonstrate the foremost specificity of the PCRP-HOMEZ-1A5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free HOMEZ antibody (clone PCRP-HOMEZ-1A5) as confirmation of integrity and purity.