GRP78 / BiP Antibody

• Catalog Number: orb1261265
• Category: Antibodies
• Description: GRP78 / BiP Antibody
• Target: HSPA5
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1).
• UniProt ID: P11021
• MW: Observed: 78kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP
• Research Area: Neuroscience
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HSPA5 antibody (orb1261265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat testis using GRP78/BiP antibody (orb1261265) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using GRP78/BiP antibody (orb1261265) at dilution of 1:100 (40x lens).