GPX4/MCSP Antibody

SKU: orb1538019

Description

GPX4/MCSP Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
• Reactivity: Human, Monkey
• Application Notes:
  Further information: Immunohistochemistry: LS-B3246 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3246 was determined to be 2.5 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EEPLVIEKDLPHY, from the C-Terminus of protein sequence according to NP_002076.2NP_001034937.1.
• Target: GPX4 / MCSP
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydroperoxidase, SnGPx, Glutathione peroxidase 4, GSHPx-4, PHGPx

Antibody (0.1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GPX4 / MCSP