Goat CD163 Antibody

SKU: orb2899324

Description

Goat CD163 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 150kDa band observed in lysates of cell line Caco-2 (calculated MW of 125kDa according to NP_004235.4). Molecular weights of 150-200kDa have been observed by other commercial sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of Caco-2 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human

Key Properties

• Host: Goat
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

CD163, CD163 molecule, M130, MM130, hemoglobin scavenger receptor, macrophage-associated antigen, scavenger receptor cysteine-rich type 1 protein M130

Primary incubation 1 hour at room temperature. Image A: Caco-2 cell lysate at primary Ab concentration 2 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Quick Database Links

RefSeq (Protein):NP_004235.4, NP_981961.2