Goat Rabbit IgG (H&L) Antibody (FITC)

SKU: orb1236937

Description

Goat Rabbit IgG (H&L) Antibody (FITC)

Images & Validation

• Tested Applications: FC, IF
• Reactivity: Rabbit
• Application Notes:
  Fluorescein Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended dilutions are as follows, Flow Cytometry 1:2,000 - 1:10,000, IF Microscopy 1:500 - 1:2,500. (Optimal dilution should be determined by user).

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Isotype: IgG
• Immunogen: Rabbit IgG - whole molecule
• Purification: Fluorescein Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
• Disclaimer: For research use only