Goat anti-Rabbit IgG Fc, HRP conjugated

SKU: orb700369

Description

Goat polyclonal antibody against Goat anti-Rabbit IgG Fc conjugated to HRP

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: The optimal working dilution should be determined by the investigator
• Reactivity: Rabbit
• Application Notes:
  This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified Rabbit IgG
• Purity: Immunogen affinity purified goat IgG.
• Purification: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Storage & Handling

• Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Form/Appearance: Lyophilized
• Disclaimer: For research use only