PRDM14 (aa414-427) Antibody

SKU: orb22534

Description

Goat polyclonal antibody to PRDM14

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution range: ELISA: 1:16000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Preliminary experiments gave an approx 40kDa band in Human, Mouse and Rat Testis lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 64.1kDa according to NP_078780.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PRDM14 (aa414-427)
• Molecular Weight: 64.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PRDM14 antibody, anti PR domain containing 14 antibody, anti MGC59730 antibody, anti PFM11 antibody, anti PR domain zinc finger protein 14 antibody, anti PR domain-containing protein 14 antibody, anti PR-domain zinc finger protein 14 antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using PRDM14 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using PRDM14 antibody.

Quick Database Links

Gene Symbol

PRDM14 (aa414-427)

NCBI Gene ID

63978