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Catalog Number | orb18401 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IRF5 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Human, Mouse, Rat |
Dilution range | ELISA: 1:128000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 56.0; 56.0; 57.8 |
Target | IRF5 |
Entrez | 3663 |
Protein Sequence | QGPWPMHPAGMQ |
RRID | AB_10755398 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IRF5 antibody, anti interferon regulatory fac Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 lysate using IRF5 antibody
Western blot analysis of Human Spleen (Lane 1) and Peripheral Blood Lymphocytes (Lane 2) lysates using IRF5 antibody