Goat anti-FXR1 Antibody

• Catalog Number: orb19295
• Category: Antibodies
• Description: Goat polyclonal antibody to FXR1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 69.7; 60.8; 59.9
• Target: FXR1
• Entrez: 8087
• Protein Sequence: RIEGDNENKLPRED
• RRID: AB_10750673
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FXR1 antibody, anti fragile X mental retardat
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19295 (0.2ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of NIH-3T3 cell lysate using FXR1 antibody

Immunofluorescence analysis of A431 cells using FXR1 antibody

Immunofluorescence analysis of HeLa cells using FXR1 antibody

Flow cytometric analysis of HeLa cells using FXR1 antibody