FXR1 Antibody

SKU: orb19295

Description

Goat polyclonal antibody to FXR1

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FXR1
• Molecular Weight: 69.7; 60.8; 59.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FXR1 antibody, anti fragile X mental retardation, autosomal homolog 1 antibody, anti fragile X mental retardation-related protein 1 antibody

orb19295 (0.2ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of NIH-3T3 cell lysate using FXR1 antibody

Immunofluorescence analysis of A431 cells using FXR1 antibody

Immunofluorescence analysis of HeLa cells using FXR1 antibody

Flow cytometric analysis of HeLa cells using FXR1 antibody

Quick Database Links

Gene Symbol

FXR1

NCBI Gene ID

8087