GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb750205
• Category: Antibodies
• Description: Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.
• UniProt ID: P14136
• Tested applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM248
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).

Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.

Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.