GFAP Antibody / Glial Fibrillary Acidic Protein Cocktail

• Catalog Number: orb749518
• Category: Antibodies
• Description: This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: GFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.
• UniProt ID: P14136
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Application notes: Optimal dilution of the GFAP antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: GA-5 + ASTRO/789
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

Western blot testing of human brain lysate with GFAP antibody cocktail (clones GA-5 + ASTRO/789). Predicted molecular weight ~50 kDa.