General Nitrotyrosine (NT) ELISA Kit

• Catalog Number: orb1994886
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NT in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Concentration: 500 ng/mL
• Application notes: standard: 500 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NT in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 7.82-500 ng/mL
• Sensitivity: 2.1 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: Nitrotyrosine
• Research Area: Infectious Disease & Virology, Metabolism Research
• Note: For research use only