General 3-Nitrotyrosine (3-NT) ELISA Kit

• Catalog Number: orb1806518
• Category: Assays and Kits
• Description: General 3-Nitrotyrosine (3-NT) ELISA Kit
• Target: 3-NT
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-Nitrotyrosine(3-NT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-Nitrotyrosine(3-NT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Nitrotyrosine(3-NT) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 300ng/mL
• Range: 4.69-300ng/mL
• Sensitivity: 1.35 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine
• Research Area: Infectious Disease & Virology, Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.