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| Catalog Number | orb781672 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hydroxyproline(Hyp) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxyproline(Hyp). |
| Reactivity | All |
| Concentration | 5000 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 5000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 78.13-5000 ng/mL |
| Sensitivity | 25.4 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | (2S,4R)-4-Hydroxyproline ELISA Kit, L-Hydroxyproli Read more... |
| Note | For research use only |
