F(ab')2 Mouse IgG (H&L) Antibody

• Catalog Number: orb346783
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit F(ab')2 Anti-MOUSE IgG Antibody, Rabbit Fab
• Note: For research use only
• Application notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
• Expiration Date: 12 months from date of receipt.

FcγRIV-dependent uptake and activation of kidney, but not dermal, macrophages by small immune complexes, ‘auto' antibodies, and NZWxBXSB serum. (H) TNF staining in kidney F4/80bright macrophages from wt mice 1 hour after i.v. injection of the indicated amounts of BSA, RaBSA, BSA;RaBSA, rabbit IgG (Ra-IgG), rabbit anti-mouse transferrin (Ra-mTransferrin) [p/n orb345374], rabbit anti-mouse albumin (Ra-mAlbumin) [p/n orb2652784], rabbit anti-mouse IgG (Ra-mIgG) [p/n orb347515] or Ra- mIgG (Fab')2 [p/n orb346783]. N = 4–5 mice per treatment.