F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin conjugated

• Catalog Number: orb348113
• Category: Antibodies
• Description: F(ab')2 Goat IgG (H&L) antibody (RPE)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: RPE
• Reactivity: Goat
• Form/Appearance: Lyophilized
• Concentration: 0.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: F(ab')2 fragment antibody Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Immunogen: Goat IgG whole molecule
• Tested applications: FC, IF
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Alternative names: Rabbit F(ab')2 Anti-Goat IgG Antibody Phycoerythri
• Note: For research use only