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F(ab')2 Chicken IgG (H&L) Antibody Texas Red Conjugated
Catalog Number: orb348097
| Catalog Number | orb348097 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Chicken IgG (H&L) antibody (Texas Red) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG F(ab')2 |
| Conjugation | Texas Red |
| Reactivity | Gallus |
| Form/Appearance | Lyophilized |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Immunogen | Chicken IgG whole molecule |
| Tested applications | DOT, IF |
| Dilution range | IF: 1:500-1:2,500 |
| Application notes | F(ab')2 Anti-Chicken IgG Texas Red Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | Rabbit F(ab')2 Anti-Chicken IgG Texas Red Conjugat Read more... |
| Note | For research use only |
Dot Blot results of Rabbit F(ab')2 Anti-Chicken IgG Antibody Texas Red™ Conjugated. Dots are Chicken IgG/IgY (p/n orb2652774) at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Antibody: Rabbit F(ab')2 Anti-Chicken IgG Antibody Texas Red™ (p/n orb348097) at 1 µg/ml for 1hr at RT.
