PTGS1 Antibody

SKU: orb1247911

Description

PTGS1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QDDGPAVERPSTEL
• Target: PTGS1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6

orb1247911 (2 ug/ml) staining of U937 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247911 (3.8 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247911 (0.5 ug/ml) staining of Mouse Kidney (A) and Skeletal Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247911 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and vesicle staining.

Quick Database Links

Gene Symbol

PTGS1

UniProt

P23219

RefSeq (Protein):NP_000953.2, NP_542158.1