CLU/Clusterin Antibody

SKU: orb1537927

Description

CLU/Clusterin Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: LS-B2937 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2937 was determined to be 10 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 1A11
• Immunogen: Recombinant human Clusterin (1-333aa) purified from E. coli.
• Target: CLU / Clusterin
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide. PBS, pH 7.4, 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40

Cell lysates of U87 MG (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Quick Database Links

Gene Symbol

CLU / Clusterin