CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody

• Catalog Number: orb11204
• Category: Antibodies
• Description: CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P27 kip1 (101-198/198aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: CDKN1B
• UniProt ID: P46527
• RRID: AB_10748049
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: p27 KIP 1; CDN1B_HUMAN; AA408329; AI843786; Cdki1b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

CDKN1B (p27Kip1) was immunoprecipitated from mouse kidney tissue with orb11204 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Goat anti-Rabbit IgG (Heavy Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: mouse kidney tissue lysate 10 µg (Input). Lane 2: orb11204 IP in mouse kidney tissue lysate. Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control). Secondary, All lanes Goat anti-Rabbit IgG (Heavy Chain specific), HRP Conjugated, 1:5000.

Sample: 293T (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-CDKN1B'p27 KIP 1 (orb11204) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.