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CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody (PE-Cy7)
Catalog Number: orb914128
| Catalog Number | orb914128 |
|---|---|
| Category | Antibodies |
| Description | CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody (PE-Cy7) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF |
| Predicted Reactivity | Canine, Gallus, Porcine, Sheep |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human P27 kip1 (101-198/198aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | Flow-Cyt=1μg/Test , IF=1:100-500 |
| Conjugation | PE/Cy7 |
| Target | CDKN1B |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | p27 KIP 1; CDN1B_HUMAN; AA408329; AI843786; Cdki1b Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-CDKN1B p27 KIP 1 Antibody, conjugated (orb914128) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
