CDK4 Rabbit Polyclonal Antibody

SKU: orb573611

Description

Rabbit polyclonal antibody to CDK4

Images & Validation

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Item 1 of 6

Tested Applications	IHC-P, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Target	CDK4
Molecular Weight	34kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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CMM3, PSK-J3

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CDK4 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with orb573611 with 1:200 dilution. Western blot was performed using orb573611 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: CDK4 IP with orb573611 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

Sample Tissue: Human A549 Whole Cell, Antibody dilution: 1 ug/ml.

Lanes: 1. 30 ug human HCT116 cell lysate, 2. 30 ug genotoxic treated human HCT116 cell lysate, 3. 30 ug genotoxic treated human HCT116 cell lysate, Primary Antibody dilution: 1:2000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody dilution: 1:5000, Gene Name: CDK4.

Lanes: Lane 1: 30 ug untreated human HCT116 cell lysate, Lane 2-3: 30 ug genotoxic agent treated human HCT116 cell lysate, Primary Antibody dilution: 1:2000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody dilution: 1:5000, Gene Name: CDK4, CDK4 is supported by BioGPS gene expression data to be expressed in HCT116.

Rabbit Anti-CDK4 antibody, Catalog Number: orb573611, Paraffin Embedded Tissue: Human Heart cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

WB Suggested Anti-CDK4 Antibody, Titration: 1.0 ug/ml, Positive Control: HepG2 Whole Cell.

Quick Database Links

Gene Symbol

CDK4

UniProt

P11802

RefSeq (Protein):NP_000066

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000066

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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